TY - JOUR TI - ComQXPA Quorum Sensing Systems May Not Be Unique to Bacillus subtilis: A Census in Prokaryotic Genomes AU - Dogsa, Iztok AU - Choudhary, Kumari Sonal AU - Marsetic, Ziva AU - Hudaiberdiev, Sanjarbek AU - Vera, Roberto AU - Pongor, Sándor AU - Mandic-Mulec, Ines T2 - PlosOne AB - The comQXPA locus of Bacillus subtilis encodes a quorum sensing (QS) system typical of Gram positive bacteria. It encodes four proteins, the ComQ isoprenyl transferase, the ComX pre-peptide signal, the ComP histidine kinase, and the ComA response regulator. These are encoded by four adjacent genes all situated on the same chromosome strand. Here we present results of a comprehensive census of comQXPA-like gene arrangements in 2620 complete and 6970 draft prokaryotic genomes (sequenced by the end of 2013). After manually checking the data for false-positive and false-negative hits, we found 39 novel com-like predictions. The census data show that in addition to B. subtilis and close relatives, 20 comQXPA-like loci are predicted to occur outside the B. subtilis clade. These include some species of Clostridiales order, but none outside the phylum Firmicutes. Characteristic gene-overlap patterns were observed in comQXPA loci, which were different for the B. subtilis-like and non-B. subtilis-like clades. Pronounced sequence variability associated with the ComX peptide in B. subtilis clade is evident also in the non-B. subtilis clade suggesting grossly similar evolutionary constraints in the underlying quorum sensing systems. DA - 2014/05/02/ PY - 2014 DO - 10.1371/journal.pone.0096122 DP - PLoS Journals VL - 9 IS - 5 SP - e96122 J2 - PlosOne LA - en SN - 1932-6203 ST - ComQXPA Quorum Sensing Systems May Not Be Unique to Bacillus subtilis UR - https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0096122 Y2 - 2020/08/06/16:28:07 KW - Bacillus cereus KW - Bacillus subtilis KW - Genetic loci KW - Genomics KW - Hidden Markov models KW - Phylogenetic analysis KW - Protein domains KW - Quorum sensing ER -